M51
(Plasmid
#242620)
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PurposeStrep-MYC-His protein expression in bacteria
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET30a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsgrowth 30°C, protein expression 12°C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStrep-MYC-His
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SpeciesH. sapiens (human)
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Tags
/ Fusion Proteins
- Strep (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: NdeI, 3' Cloning Site: XhoI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M51 was a gift from Alain Nepveu (Addgene plasmid # 242620 ; http://n2t.net/addgene:242620 ; RRID:Addgene_242620)