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M55
(Plasmid #242623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242623 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET30a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    growth 30°C, protein expression 12°C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Strep-Flag-MYCMutMBIV-His
  • Species
    H. sapiens (human)
  • Mutation
    A310E,A311D,P312G,P313G,R315A,K316A,D317S
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tags / Fusion Proteins
    • Strep (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: NdeI, 3' Cloning Site: XhoI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M55 was a gift from Alain Nepveu (Addgene plasmid # 242623 ; http://n2t.net/addgene:242623 ; RRID:Addgene_242623)

  • For your References section:

    The function of MYC in base excision repair protects against RAS-induced senescence. Faraco CCF, Zhu W, Fortier AM, Ramdzan ZM, Vickridge E, Ong V, Goudreau F, Djerir B, Bellemare J, Gu S, Zhang H, Carroll BL, Krishnan SS, Leduy L, Guarne A, Park M, Marechal A, Nepveu A. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf706. doi: 10.1093/nar/gkaf706. 10.1093/nar/gkaf706 PubMed 40737085
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