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Addgene

M77
(Plasmid #242629)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242629 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pCF806
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-MYCMutMBIIIa, MutMBIV
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Mutation
    I189A,V193A,V194A,F195S,L199A,A310E,A311D,P312G,P313G,R315A,K316A,D317S
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • Supplemental Documents

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: XhoI, 3' Cloning Site: EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M77 was a gift from Alain Nepveu (Addgene plasmid # 242629 ; http://n2t.net/addgene:242629 ; RRID:Addgene_242629)