M77
(Plasmid
#242629)
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PurposeMYC knockdown + rescue
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCF806
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-MYCMutMBIIIa, MutMBIV
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gRNA/shRNA sequenceUnspecified
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SpeciesH. sapiens (human)
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MutationI189A,V193A,V194A,F195S,L199A,A310E,A311D,P312G,P313G,R315A,K316A,D317S
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: XhoI, 3' Cloning Site: EcoRI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M77 was a gift from Alain Nepveu (Addgene plasmid # 242629 ; http://n2t.net/addgene:242629 ; RRID:Addgene_242629)