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pplss-mRFP-KDEL-pcDNA3.1/Hygro
(Plasmid #242808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242808 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro
  • Total vector size (bp) 6400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP with ER targeting signal sequence and C-terminal KDEL-ER retention signal
  • gRNA/shRNA sequence
    none
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert (prlSS-mRFP-KDEL) was derived from Eric A. Snapp. Original reference: Altan-Bonnet et al. DOI 10.1091/mbc.e05-02-0155.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please cite original reference: Altan-Bonnet et al. DOI 10.1091/mbc.e05-02-0155.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pplss-mRFP-KDEL-pcDNA3.1/Hygro was a gift from Christoph Kaether (Addgene plasmid # 242808 ; http://n2t.net/addgene:242808 ; RRID:Addgene_242808)
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