Skip to main content
Addgene

TRE-RXRa-Myc; pGK-H2B-RFP
(Plasmid #242887)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242887 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    TRC
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8909
  • Modifications to backbone
    The pLKO vector was modified in the Fuchs lab to contain the inducible tetracycline response element at the 5′ end, as well as a 3′ MYC epitope tag (Gur-Cohen, S. et al. Science, 2019).
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rxra
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1374
  • Mutation
    Human RXRa cDNA was PCR-amplified from pSV-Sport-RXRα (a gift from B. Spiegelman; Addgene #8882) and a NheI site was added 5'.
  • GenBank ID
    6256
  • Entrez Gene
    RXRA (a.k.a. NR2B1, RXR-alpha, RXRalpha)
  • Promoter Tetracycline response element upstream of a minimal CMV promoter
  • Tag / Fusion Protein
    • H2B-mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    B. Spiegelman (pSV-Sport-RXRα; Addgene #8882)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE-RXRa-Myc; pGK-H2B-RFP was a gift from Elaine Fuchs (Addgene plasmid # 242887 ; http://n2t.net/addgene:242887 ; RRID:Addgene_242887)

  • For your References section:

    Stem cells tightly regulate dead cell clearance to maintain tissue fitness. Stewart KS, Abdusselamoglu MD, Tierney MT, Gola A, Hur YH, Gonzales KAU, Yuan S, Bonny AR, Yang Y, Infarinato NR, Cowley CJ, Levorse JM, Pasolli HA, Ghosh S, Rothlin CV, Fuchs E. Nature. 2024 Sep;633(8029):407-416. doi: 10.1038/s41586-024-07855-6. Epub 2024 Aug 21. 10.1038/s41586-024-07855-6 PubMed 39169186
Related items: