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pLenti-THP-(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
(Plasmid #242904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242904 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    plenti.THP
  • Backbone size w/o insert (bp) 8752
  • Total vector size (bp) 10381
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
  • Species
    Synthetic
  • Insert Size (bp)
    1629
  • Promoter rat Tyrosine hydroxylase

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PsPXI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer GCCACCATGAAGACTATTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kwang-Soo Kim Lab (Addgene #80335, THP)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more info about iATPSnFR2 sensor: https://doi.org/10.1073/pnas.2314604121

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-THP-(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3 was a gift from Timothy Ryan (Addgene plasmid # 242904 ; http://n2t.net/addgene:242904 ; RRID:Addgene_242904)

  • For your References section:

    Neuromodulatory control of energy reserves in dopaminergic neurons. Pulido C, Gentry MS, Ryan TA. Proc Natl Acad Sci U S A. 2025 Dec 16;122(50):e2523019122. doi: 10.1073/pnas.2523019122. Epub 2025 Dec 5. 10.1073/pnas.2523019122 PubMed 41348744
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