pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3-H
(Plasmid #242971)

• Purpose: Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3-H FPs for ratiometric analysis
• Depositing Lab: Dorus Gadella
• Publication: mScarlet3-H plasmids (unpublished)

Backbone

• Vector backbone: pTriEx-3
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet3-H
• Species: Other
• Promoter: CMV & Rha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry