IronFistMUT.
(Plasmid #243008)

• Purpose: Iron binding deficient IronFist
• Depositing Lab: Roland Malli
• Publication: Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. doi: 10.1021/acssensors.5c01165. Epub 2025 Aug 1.

Backbone

• Vector backbone: Vector Builder
• Backbone manufacturer: Vector Builder
• Total vector size (bp): 4827
• Vector type: Mammalian Expression ; Gateway

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: hemerythrin-like-mNeonGreen
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: H15A, H57A, E58A, E61A, H126A, E130A
• Entrez Gene: FBXL5 (a.k.a. FBL4, FBL5, FLR1)
• Promoter: human phosphoglycerate kinase (hPGK)
• Tag / Fusion Protein:
  • mNeonGreen (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: cctataaaaataggcgtatc

Gene/Insert 2

• Gene/Insert name: T2A
• Alt name: Ribosomal skipping sequence
• Insert Size (bp): 63

Gene/Insert 3

• Gene/Insert name: mCherry
• Insert Size (bp): 711

Resource Information

• Supplemental Documents:
  • IronFistMUT.gbk
• A portion of this plasmid was derived from a plasmid made by: We have ordered it from Vector Builder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry