|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24356||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)3000
Entrez GenelacZ (a.k.a. ECO111_0380)
/ Fusion Protein
- 8 aa nuclear localization signal (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer LacZ-F (Common Sequencing Primers)
The nuclear LacZ insert was PCR amplified from UAS-nucLacZ genomic flies (Bloomington Stock Center). The KpnI/XbaI digested nucLacZ PCR fragment was then
ligated into pQUAST.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQUAST-nucLacZ was a gift from Liqun Luo (Addgene plasmid # 24356 ; http://n2t.net/addgene:24356 ; RRID:Addgene_24356)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990