6xHis-FUS-LCdel
(Plasmid
#243671)
-
PurposeExpresses 6xHis-tagged FUS with LC domain deletion in bacteria
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 243671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepJ411
- Backbone size w/o insert (bp) 4400
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFUS-delLC
-
SpeciesH. sapiens (human)
-
Mutationdelete LC domain
-
Entrez GeneFUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
-
Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
6xHis-FUS-LCdel was a gift from Nicolas Fawzi (Addgene plasmid # 243671 ; http://n2t.net/addgene:243671 ; RRID:Addgene_243671) -
For your References section:
Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads. Murthy AC, Tang WS, Jovic N, Janke AM, Seo DH, Perdikari TM, Mittal J, Fawzi NL. Nat Struct Mol Biol. 2021 Nov;28(11):923-935. doi: 10.1038/s41594-021-00677-4. Epub 2021 Nov 10. 10.1038/s41594-021-00677-4 PubMed 34759379