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MBP-FUS-RGG3
(Plasmid #243672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243672 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTHMT
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FUS-RGG3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    180
  • Mutation
    WT
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Tag / Fusion Protein
    • Maltose binding protein (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-FUS-RGG3 was a gift from Nicolas Fawzi (Addgene plasmid # 243672 ; http://n2t.net/addgene:243672 ; RRID:Addgene_243672)

  • For your References section:

    Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads. Murthy AC, Tang WS, Jovic N, Janke AM, Seo DH, Perdikari TM, Mittal J, Fawzi NL. Nat Struct Mol Biol. 2021 Nov;28(11):923-935. doi: 10.1038/s41594-021-00677-4. Epub 2021 Nov 10. 10.1038/s41594-021-00677-4 PubMed 34759379
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