pJL163
(Plasmid
#243686)
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PurposeStably expresses ALDH18A1 CDS with its endogenous 5'UTR and 3'UTR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 243686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJL130/pjjca526
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUTRs and CDS of ALDH18A1
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SpeciesH. sapiens (human)
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Entrez GeneALDH18A1 (a.k.a. ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL163 was a gift from Jonathan Weissman (Addgene plasmid # 243686 ; http://n2t.net/addgene:243686 ; RRID:Addgene_243686) -
For your References section:
Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation. Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS. Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002. 10.1016/j.cell.2025.08.002 PubMed 40876456