TVMV-TEV-HRV-3C Proteases-mCherry
(Plasmid
#243798)
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PurposeEncodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 243798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTVMV, TEV and HRV-3C proteases
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MGSS-TVMV-GS(x2)-P2A-GS(x2)- SuperTEV-GS(x2)-P2A-GS(x2)-HRV-3C-GS(x2)-P2A-GS(x2)-mCherry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TVMV-TEV-HRV-3C Proteases-mCherry was a gift from Cole DeForest (Addgene plasmid # 243798 ; http://n2t.net/addgene:243798 ; RRID:Addgene_243798) -
For your References section:
Boolean logic-gated protein presentation through autonomously compiled molecular topology. Gharios R, Ross ML, Li A, Kottantharayil SP, Hoye J, DeForest CA. Nat Chem Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5. 10.1038/s41589-025-02037-5 PubMed 41068299