pGEP116[EF1a-mNeonGreen]-pAAVS1-SA-Neo-TRE3Gs-NGN2-2A-Zeo-CAG-TETON3G
(Plasmid
#243805)
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PurposeIntegrates dox-inducible hNGN2 into the human AASV1 safe harbor locus for directed differentiation of neural cells from stem cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 243805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGEP116
- Total vector size (bp) 13366
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Modifications to backboneEF1a-mNeonGreen
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Vector typeTALEN
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Selectable markersNeomycin (select with G418), Zeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNGN2
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SpeciesH. sapiens (human)
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Insert Size (bp)5945
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Entrez GeneNEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
- Promoter TRE3GS
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEP116[EF1a-mNeonGreen]-pAAVS1-SA-Neo-TRE3Gs-NGN2-2A-Zeo-CAG-TETON3G was a gift from Michael Wells (Addgene plasmid # 243805 ; http://n2t.net/addgene:243805 ; RRID:Addgene_243805)