Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24382||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2038
MutationT179A, S399A, S413A, S555A, S588A, and S626A
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
The wild-type version of FOXO3 is also available at Addgene, plasmid 8360.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-FOXO3 6A was a gift from Anne Brunet (Addgene plasmid # 24382 ; http://n2t.net/addgene:24382 ; RRID:Addgene_24382)
For your References section:The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor. Greer EL, Oskoui PR, Banko MR, Maniar JM, Gygi MP, Gygi SP, Brunet A. J Biol Chem. 2007 Oct 12. 282(41):30107-19. 10.1074/jbc.M705325200 PubMed 17711846