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mito_mCherry_ALFA
(Plasmid #244043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244043 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA4TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CISD1-mCherry-ALFA
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • ALFA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mito_mCherry_ALFA was a gift from Markus Affolter (Addgene plasmid # 244043 ; http://n2t.net/addgene:244043 ; RRID:Addgene_244043)

  • For your References section:

    Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster. Vigano MA, Ell CM, Kustermann MMM, Aguilar G, Matsuda S, Zhao N, Stasevich TJ, Affolter M, Pyrowolakis G. Development. 2021 Mar 16;148(6):dev191700. doi: 10.1242/dev.191700. 10.1242/dev.191700 PubMed 33593816
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