mCherry_CD8_OLLAS_SunTag
(Plasmid
#244060)
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Purposeanchoring 1xOLLAS-SunTag to membranes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepTwist
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry_CD8_OLLAS_SunTag
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- OLLAS-SunTagV4 (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry_CD8_OLLAS_SunTag was a gift from Markus Affolter (Addgene plasmid # 244060 ; http://n2t.net/addgene:244060 ; RRID:Addgene_244060) -
For your References section:
Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster. Vigano MA, Ell CM, Kustermann MMM, Aguilar G, Matsuda S, Zhao N, Stasevich TJ, Affolter M, Pyrowolakis G. Development. 2021 Mar 16;148(6):dev191700. doi: 10.1242/dev.191700. 10.1242/dev.191700 PubMed 33593816