pCAG-CaBLAM
(Plasmid
#244129)
-
PurposeBioluminescent calcium indicator
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepCAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCaBLAM
-
SpeciesSynthetic
-
MutationCalmodulin N98I
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.06.25.546478 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAG-CaBLAM was a gift from Nathan Shaner (Addgene plasmid # 244129 ; http://n2t.net/addgene:244129 ; RRID:Addgene_244129) -
For your References section:
CaBLAM: a high-contrast bioluminescent Ca(2+) indicator derived from an engineered Oplophorus gracilirostris luciferase. Lambert GG, Crespo EL, Murphy J, Turner KL, Gershowitz E, Cunningham M, Boassa D, Luong S, Celinskis D, Allen JJ, Venn S, Zhu Y, Karadas M, Chen J, Marisca R, Gelnaw H, Nguyen DK, Hu J, Sprecher BN, Tree MO, Orcutt R, Heydari D, Bell AB, Torreblanca-Zanca A, Hakimi A, Czopka T, Shoham S, Nagel KI, Schoppik D, Andrade A, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC. Nat Methods. 2025 Dec 2. doi: 10.1038/s41592-025-02972-0. 10.1038/s41592-025-02972-0 PubMed 41331138