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pCAG-CaBLAM_332W
(Plasmid #244131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244131 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaBLAM_332W
  • Species
    Synthetic
  • Mutation
    Calmodulin Q135W

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are minor discrepancies in the CAG promoter - these are confirmed by the depositor to not be of concern for plasmid function.

Please visit https://doi.org/10.1101/2023.06.25.546478 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-CaBLAM_332W was a gift from Nathan Shaner (Addgene plasmid # 244131 ; http://n2t.net/addgene:244131 ; RRID:Addgene_244131)

  • For your References section:

    CaBLAM: a high-contrast bioluminescent Ca(2+) indicator derived from an engineered Oplophorus gracilirostris luciferase. Lambert GG, Crespo EL, Murphy J, Turner KL, Gershowitz E, Cunningham M, Boassa D, Luong S, Celinskis D, Allen JJ, Venn S, Zhu Y, Karadas M, Chen J, Marisca R, Gelnaw H, Nguyen DK, Hu J, Sprecher BN, Tree MO, Orcutt R, Heydari D, Bell AB, Torreblanca-Zanca A, Hakimi A, Czopka T, Shoham S, Nagel KI, Schoppik D, Andrade A, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC. Nat Methods. 2025 Dec 2. doi: 10.1038/s41592-025-02972-0. 10.1038/s41592-025-02972-0 PubMed 41331138
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