L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreen
(Plasmid
#244170)
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PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): hCD8aSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepPD1054 Polytransfect L1 mNeonGreen
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMESA NTEVp chain with human CD8a SS, human IL-10Rb ECD and TMD, and NTEVp (75S)
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SpeciesH. sapiens (human), Synthetic
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MutationNTEVp_75S
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Entrez GeneIL10RB (a.k.a. CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2)
- Promoter hEF1a
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Tag
/ Fusion Protein
- human CD8a signal peptide 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.23.586421 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreen was a gift from Joshua Leonard (Addgene plasmid # 244170 ; http://n2t.net/addgene:244170 ; RRID:Addgene_244170) -
For your References section:
Conversion of natural cytokine receptors into orthogonal synthetic biosensors. Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN. Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. 10.1038/s41589-025-01986-1 PubMed 40846994