SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3
(Plasmid
#244224)
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Purpose(Empty Backbone) Vector for cloning LbCas12a crRNA array libraries. Contains 3' TruSeq sequence for easy NGS library construction
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepHAGE
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.08.602263 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3 was a gift from Tim Reddy (Addgene plasmid # 244224 ; http://n2t.net/addgene:244224 ; RRID:Addgene_244224) -
For your References section:
HyperCas12a enables highly-multiplexed epigenome editing screens. Melore SM, Hamilton MC, Reddy TE. bioRxiv [Preprint]. 2024 Jul 9:2024.07.08.602263. doi: 10.1101/2024.07.08.602263. 10.1101/2024.07.08.602263 PubMed 39026853