pGEM-T_mouseH3.1-HaloTag
(Plasmid
#244242)
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PurposeCRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGem-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5080
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH3.1
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Alt nameHist1h3a
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SpeciesM. musculus (mouse)
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Insert Size (bp)2104
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Entrez GeneH3c1 (a.k.a. H3c10, H3c11, H3c8, Hist1h3a)
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Tag
/ Fusion Protein
- HaloTag (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-T_mouseH3.1-HaloTag was a gift from Kazuhiro Maeshima (Addgene plasmid # 244242 ; http://n2t.net/addgene:244242 ; RRID:Addgene_244242) -
For your References section:
Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells. Minami K, Nakazato K, Ide S, Kaizu K, Higashi K, Tamura S, Toyoda A, Takahashi K, Kurokawa K, Maeshima K. Sci Adv. 2025 Mar 28;11(13):eadu8400. doi: 10.1126/sciadv.adu8400. Epub 2025 Mar 28. 10.1126/sciadv.adu8400 PubMed 40153514