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pADH99Cau
(Plasmid #244807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244807 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pADH99
  • Backbone manufacturer
    Namkha Nguyen
  • Backbone size w/o insert (bp) 2226
  • Total vector size (bp) 11734
  • Modifications to backbone
    Changed the C. albicans HIS1 promoter and ENO1 promoter sequences with the C. auris ones.
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    100µg/mL Ampicillin
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CauHIS1_US
  • Species
    C. auris
  • Insert Size (bp)
    1447
  • Tags / Fusion Proteins
    • C. albicans codon optimized Cas9
    • C. albicans MAL2 promoter
    • S. cerevisiae FLP recombinase
    • NAT resistance (first half)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.09.632232 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADH99Cau was a gift from Patrick Van Dijck (Addgene plasmid # 244807 ; http://n2t.net/addgene:244807 ; RRID:Addgene_244807)

  • For your References section:

    A comparative evaluation of CRISPR-Cas9 allele editing systems in Candida auris: challenging research in a challenging bug. Sofras D, Carolus H, Subotic A, Romero CL, Ennis CL, Hernday AD, Nobile CJ, Rybak JM, Van Dijck P. bioRxiv [Preprint]. 2025 Jan 11:2025.01.09.632232. doi: 10.1101/2025.01.09.632232. 10.1101/2025.01.09.632232 PubMed 39829791
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