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pAttBF3Hyg
(Plasmid #244885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244885 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript II derivative
  • Backbone size (bp) 2850
  • Modifications to backbone
    Addition of an FRT3 site, a Multiple Csloning Site with SapI sites for Golden Gate cloning, a lox2722 flanked rps-0 promoter HygR unc-54 3' UTR cassette, and a phiC31 attB site.
  • Vector type
    Worm Expression ; phiC31 recombinase mediated insertion vector
  • Promoter none
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAttBF3Hyg was a gift from Michael Nonet (Addgene plasmid # 244885 ; http://n2t.net/addgene:244885 ; RRID:Addgene_244885)

  • For your References section:

    A single component landing site for efficient transgenesis using recombination-mediated insertion. Beck AA, Nonet ML. microPublication Biology 10.17912/micropub.biology.001784
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