SIN-cPPT-G1B3-mitoGO-ATeam2-WPRE-miR124T
(Plasmid
#245075)
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PurposeMitochondrial ATP biosensor, with miRNA-124 target sequence to repress transgene expression in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneSIN-LV
- Backbone size w/o insert (bp) 7739
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitoGO-ATeam2
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SpeciesSynthetic
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Insert Size (bp)4144
- Promoter Human G1B3 promoter
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIN-cPPT-G1B3-mitoGO-ATeam2-WPRE-miR124T was a gift from Nicole Déglon (Addgene plasmid # 245075 ; http://n2t.net/addgene:245075 ; RRID:Addgene_245075) -
For your References section:
Tau accumulation in astrocytes of the dentate gyrus induces neuronal dysfunction and memory deficits in Alzheimer's disease. Richetin K, Steullet P, Pachoud M, Perbet R, Parietti E, Maheswaran M, Eddarkaoui S, Begard S, Pythoud C, Rey M, Caillierez R, Q Do K, Halliez S, Bezzi P, Buee L, Leuba G, Colin M, Toni N, Deglon N. Nat Neurosci. 2020 Dec;23(12):1567-1579. doi: 10.1038/s41593-020-00728-x. Epub 2020 Nov 9. 10.1038/s41593-020-00728-x PubMed 33169029