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pHTN-HaloTagCMV-CLIP-170
(Plasmid #245359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245359 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHTN-HaloTag CMV-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6143
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLIP1 isofom b
  • Alt name
    CLIP-170
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4236
  • Entrez Gene
    CLIP1 (a.k.a. CLIP, CLIP-170, CLIP170, CYLN1, RSN)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a D1359E mutation within the insert. It is not known how this mutation impacts plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHTN-HaloTagCMV-CLIP-170 was a gift from Thomas Martin (Addgene plasmid # 245359 ; http://n2t.net/addgene:245359 ; RRID:Addgene_245359)
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