pJD849
(Plasmid #245490)

• Purpose: Binary Plasmid for plant transformation expressing RUBY from the 35S promoter and the terminator from the Arabidopsis thaliana HSP gene. Kanamycin plant selection
• Depositing Lab: Beth Rowan
• Publication: Monitoring the stability of transgene expression in lettuce using the RUBY reporter. (unpublished)

Backbone

• Vector backbone: 35S:RUBY Plasmid #160908
• Backbone manufacturer: Yunde Zhao
• Backbone size w/o insert (bp): 14335
• Total vector size (bp): 14394
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p35S-RUBY
• Insert Size (bp): 4891
• Promoter: 35S

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: TGAGACTTTTCAACAAAGG

Resource Information

• Supplemental Documents:
  • pjd849.gb
• A portion of this plasmid was derived from a plasmid made by: I received this plasmid from Juan Debernardi.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is derived from the 35S::RUBY plasmid submitted by Yunde Zhao (Plasmid #160908). The hygromycin plant selection marker was replaced with a kanamycin plant selection marker.