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pMAL-c2X_MBP-LcgcN(C24S)-H6
(Plasmid #245527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245527 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMAL-c2X
  • Backbone size w/o insert (bp) 6645
  • Total vector size (bp) 7014
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
  • Alt name
    LcgcN
  • Species
    metagenomic
  • Insert Size (bp)
    360
  • Mutation
    C24S within the N-terminal fragment of the LCGC14 intein
  • GenBank ID
    LAZR01010553.1
  • Promoter tac
  • Tags / Fusion Proteins
    • Maltose Binding Protein (MBP) (N terminal on insert)
    • Hexahistidine Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer malE
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-c2X_MBP-LcgcN(C24S)-H6 was a gift from Henning Mootz (Addgene plasmid # 245527 ; http://n2t.net/addgene:245527 ; RRID:Addgene_245527)

  • For your References section:

    Three-Segment Protein Labeling Using a Highly Efficient and Cysteine-Less Split Intein Identified with Computational Prediction of Aggregation Properties. Humberg C, Kroger J, Pietrokovski S, Mootz HD. Angew Chem Int Ed Engl. 2025 Oct 23:e202515821. doi: 10.1002/anie.202515821. 10.1002/anie.202515821 PubMed 41131872
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