Skip to main content
Addgene
Open Menu

snuABE4.2
(Plasmid #246014)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246014 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
  • Species
    Pediculus humanus
  • Insert Size (bp)
    -2
  • Mutation
    E438Q, I536S, E637F, with 10 amino acids truncation
  • GenBank ID
    EEB15288.1 XM_002427981.1
  • Promoter CMV
  • Tag / Fusion Protein
    • MLH1-SB (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7-forward
  • 3′ sequencing primer BGH-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.19.664718 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    snuABE4.2 was a gift from Sangsu Bae (Addgene plasmid # 246014 ; http://n2t.net/addgene:246014 ; RRID:Addgene_246014)

  • For your References section:

    Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects. Im HW, Jeong B, Lee Y, Oh YE, CJung C, Kim YW, Uhm H, Bae S. bioRxiv 2025.07.19.664718 10.1101/2025.07.19.664718
Related items: