AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2
(Plasmid
#246051)
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PurposeExpression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrVA1_cpGR2-S103ΔELS
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SpeciesSynthetic
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MutationΔELS
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2 was a gift from Dominik Niopek (Addgene plasmid # 246051 ; http://n2t.net/addgene:246051 ; RRID:Addgene_246051) -
For your References section:
A versatile anti-CRISPR platform for opto- and chemogenetic control of CRISPR-Cas9 and Cas12 across a wide range of orthologs. Brenker L, Aschenbrenner S, Bubeck F, Staykov K, Gebhardt C, Wolf B, Jendrusch M, Kroll AS, Becker J, Ambiel I, Fackler OT, Grimm D, Mathony J, Niopek D. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf752. doi: 10.1093/nar/gkaf752. 10.1093/nar/gkaf752 PubMed 40794863