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pcDNA3.1-CMV-Vega
(Plasmid #246323)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246323 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5595
  • Total vector size (bp) 7925
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vega
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATGGCTGACGTGGAAACCG
  • 3′ sequencing primer TTACACCTCGTTCTCGTAGCAGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.29.656768 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CMV-Vega was a gift from Peng Zou (Addgene plasmid # 246323 ; http://n2t.net/addgene:246323 ; RRID:Addgene_246323)
  • For your References section:

    A photostable genetically encoded voltage indicator for imaging neural activities in tissue and live animals. Cao C, Ruixin Zhu, Zhou S, Zhao Z, Lin C, Liu S, Peng L, Subach FV, Piatkevich KD, Zou P. bioRxiv 2025.05.29.656768 10.1101/2025.05.29.656768