pMV0067 [15ARE23bp -p(cFos)-fLuc-UBC-rLuc-P2A-GFP]
(Plasmid
#246421)
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PurposeFirefly luciferase driven by an AHR responsive minimal cFOS promoter and a renilla luciferase and GFP driven by a constitutive UBC promoter flipped with respect to the lentiviral backbone
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 246421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepMV0021
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameFirefly luciferase
- Promoter AHR responsive minimal cFOS promoter
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGTGTAGGTGGTGTTCAG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameRenilla luciferase-P2A-EGFP
- Promoter UbC
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer gccaccatggcttccaagg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.09.15.676391 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMV0067 [15ARE23bp -p(cFos)-fLuc-UBC-rLuc-P2A-GFP] was a gift from Marco Jost (Addgene plasmid # 246421 ; http://n2t.net/addgene:246421 ; RRID:Addgene_246421) -
For your References section:
Systematic identification and characterization of regulators of aryl hydrocarbon receptor signaling. Verma M, Desai K, Ding Y, Wang X, Bae M, Rakoff-Nahoum S, Balskus EP, Fischbach MA, Jost M. bioRxiv 2025.09.15.676391 10.1101/2025.09.15.676391