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pHL047 [15ARE23bp -p(TATA)-fLuc-UBC-rLuc-P2A-GFP]
(Plasmid #246422)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246422 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pMV0067
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Firefly luciferase
  • Promoter AHR responsive minimal promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Renilla luciferase-P2A-EGFP
  • Promoter UbC

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.15.676391 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL047 [15ARE23bp -p(TATA)-fLuc-UBC-rLuc-P2A-GFP] was a gift from Marco Jost (Addgene plasmid # 246422 ; http://n2t.net/addgene:246422 ; RRID:Addgene_246422)
  • For your References section:

    Systematic identification and characterization of regulators of aryl hydrocarbon receptor signaling. Verma M, Desai K, Ding Y, Wang X, Bae M, Rakoff-Nahoum S, Balskus EP, Fischbach MA, Jost M. bioRxiv 2025.09.15.676391 10.1101/2025.09.15.676391