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pT7-hMLH1dn (mouse) for IVT
(Plasmid #246485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246485 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pT7
  • Modifications to backbone
    The T7 promoter in these plasmids is inactivated (to prevent T7 from using the plasmid as a template). To generate the final, linear dsDNA template for IVT, use a forward primer correcting the T7 promoter and a reverse primer that adds the polyA tail.
  • Vector type
    Template for in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse MLH1dn
  • Alt name
    mus musculus MLH1 del758-760
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2271
  • Mutation
    deletion of residues 758-760
  • Entrez Gene
    Mlh1 (a.k.a. 1110035C23Rik)
  • Promoter T7

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer BGH-reverse
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Peter Chen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-hMLH1dn (mouse) for IVT was a gift from David Liu (Addgene plasmid # 246485 ; http://n2t.net/addgene:246485 ; RRID:Addgene_246485)

  • For your References section:

    Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR. Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. 10.1016/j.cell.2021.09.018 PubMed 34653350
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