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pCW-BAP-RECK-Blast
(Plasmid #246558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246558 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCW57.1
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RECK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2961
  • Entrez Gene
    RECK (a.k.a. ST15)
  • Promoter tight TRE
  • Tag / Fusion Protein
    • BAP/AviTag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Incyte cDNA clone LIFESEQ4097514

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PGK-Blast-T2A-rtTA-advanced cassette on backbone

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-BAP-RECK-Blast was a gift from Mario Vallon (Addgene plasmid # 246558 ; http://n2t.net/addgene:246558 ; RRID:Addgene_246558)

  • For your References section:

    WNT7A/B assemble a GPR124-RECK-LRP5/6 co-receptor complex to activate beta-catenin signaling in brain endothelial cells. Heiden R, Hannig L, Kuo CJ, Ergun S, Braunger BM, Vallon M. J Biol Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682. 10.1016/j.jbc.2025.110682 PubMed 40914247
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