Lifeact-mScarlet3-S2
(Plasmid #246873)

• Purpose: Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging.
• Depositing Lab: Pingyong Xu
• Publication: Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s41592-025-02962-2.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3973
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet3-S2
• Species: Synthetic
• Insert Size (bp): 687
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age_ (not destroyed)
• 3′ cloning site: Not_ (destroyed during cloning)
• 5′ sequencing primer: CMV-Forward
• 3′ sequencing primer: SV40 polyA-Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Lifeact sequence was fused in-frame with mScarlet3-S2 and inserted into the Age_/Not_ sites of pEGFP-N1.