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pET28a-NanoBondy 2H5(R72C)
(Plasmid #247051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247051 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6526
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NanoBondy 2H5(R72C)-Ctag
  • Species
    Synthetic
  • Insert Size (bp)
    1275
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • C-tag (C terminal on insert)
    • SpyTag003

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains a loop-internal SpyTag003 before the reactive SPM sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-NanoBondy 2H5(R72C) was a gift from Omer Dushek (Addgene plasmid # 247051 ; http://n2t.net/addgene:247051 ; RRID:Addgene_247051)

  • For your References section:

    NanoBondy Reaction through NeissLock Anhydride Allows Covalent Immune Cell Decoration. Vankayala LR, Adoni KR, Lim SYT, Dam T, Dushek O, Thalassinos K, Howarth MR. Bioconjug Chem. 2026 Feb 18;37(2):281-292. doi: 10.1021/acs.bioconjchem.5c00519. Epub 2026 Jan 24. 10.1021/acs.bioconjchem.5c00519 PubMed 41578972
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