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pEf1a-superDn29-dCas9-P2A-GFP
(Plasmid #247156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247156 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCB212
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    superDn29-dCas9-P2A-GFP
  • gRNA/shRNA sequence
    AGTGTGTATTCTGCTGTTGT
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341Q/L388P/Y404C
  • Promoter Ef1a
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.01.621560 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEf1a-superDn29-dCas9-P2A-GFP was a gift from Patrick Hsu (Addgene plasmid # 247156 ; http://n2t.net/addgene:247156 ; RRID:Addgene_247156)

  • For your References section:

    Site-specific DNA insertion into the human genome with engineered recombinases. Fanton A, Bartie LJ, Martins JQ, Tran VQ, Goudy L, Durrant MG, Wei J, Pawluk A, Konermann S, Marson A, Gilbert LA, Hsu PD. bioRxiv 2024.11.01.621560 10.1101/2024.11.01.621560
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