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DHFR-TEV (integrating)
(Plasmid #247218)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247218 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Custom backbone constructed with the MoClo yeast toolkit (YTK)
  • Backbone manufacturer
    John Dueber, Addgene kit #1000000061
  • Total vector size (bp) 7232
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DHFR-TEV
  • Promoter TEF2

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with NotI before transformation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DHFR-TEV (integrating) was a gift from Johannes Herrmann (Addgene plasmid # 247218 ; http://n2t.net/addgene:247218 ; RRID:Addgene_247218)

  • For your References section:

    The IQ-compete assay for measuring mitochondrial protein import efficiencies in living yeast cells. Hoffman Y, Egeler A, Rodl S, Herrmann JM. FEBS Lett. 2025 Oct 25. doi: 10.1002/1873-3468.70206. 10.1002/1873-3468.70206 PubMed 41137490
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