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LvPolyubiquitin-roGFP2-ORP1-minos-lox-ITR
(Plasmid #247293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247293 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist
  • Backbone size w/o insert (bp) 2971
  • Total vector size (bp) 7945
  • Vector type
    Synthetic Biology ; Sea urchin expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    roGFP2-ORP1
  • Species
    Synthetic
  • Insert Size (bp)
    1311
  • Promoter Polyubiquitin
  • Tag / Fusion Protein
    • Grx1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCTACCTTAATCTCAAGAAGAGCAAAACAAAAGC
  • 3′ sequencing primer CTTAAAACAATAATTTAATTAATTTCCCCCGCGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    roGFP2-ORP1 is derived from pUAST cyto roGFP2-Orp1 (Addgene plasmid#64996 by Tobias Dick)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LvPolyubiquitin-roGFP2-ORP1-minos-lox-ITR was a gift from Amro Hamdoun (Addgene plasmid # 247293 ; http://n2t.net/addgene:247293 ; RRID:Addgene_247293)
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