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pX330-AAVS1
(Plasmid #247356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247356 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pX330
  • Backbone manufacturer
    Feng Zhang lab (Addgene plasmid # 42230)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAVS1
  • Alt name
    sgRNA targeting human AAVS1 site
  • gRNA/shRNA sequence
    5′-GGCCACTAGGGACAGGAT-3′
  • Species
    H. sapiens (human)
  • Entrez Gene
    AAVS1 (a.k.a. AAV)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-AAVS1 was a gift from Kazuhiro Maeshima (Addgene plasmid # 247356 ; http://n2t.net/addgene:247356 ; RRID:Addgene_247356)

  • For your References section:

    Transcriptional suppression of ribosomal DNA with phase separation. Ide S, Imai R, Ochi H, Maeshima K. Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct. 10.1126/sciadv.abb5953 PubMed 33055158
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