Skip to main content
Addgene
Open Menu

pPB-EF1α-IB-H2B-HaloTag
(Plasmid #247449)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247449 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pPB-CAG-IB-H2B-PA-mCherry
  • Backbone manufacturer
    Maeshima Lab (Addgene #247339)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B (H2BC11)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1928
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter EF1α
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-EF1α-IB-H2B-HaloTag was a gift from Kazuhiro Maeshima (Addgene plasmid # 247449 ; http://n2t.net/addgene:247449 ; RRID:Addgene_247449)

  • For your References section:

    Chromatin organization and behavior in HRAS-transformed mouse fibroblasts. Otsuka A, Minami K, Higashi K, Kawaguchi A, Tamura S, Ide S, Hendzel MJ, Kurokawa K, Maeshima K. Chromosoma. 2024 Apr;133(2):135-148. doi: 10.1007/s00412-024-00817-x. Epub 2024 Feb 24. 10.1007/s00412-024-00817-x PubMed 38400910
Related items:
Commonly requested with: