pX330_H1.0
(Plasmid
#247482)
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PurposeExpresses SpCas9 and a sgRNA targeting the human histone H1.0 loci for knock-in.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepX330
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Backbone manufacturerFeng Zhang lab (Addgene plasmid # 42230)
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH1.0 (H1F0)
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Alt namesgRNA targeting human H1.0 (H1F0)
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gRNA/shRNA sequenceCACCGAGCAAAGTCCAGTGCCAAGA
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SpeciesH. sapiens (human)
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Entrez GeneH1-0 (a.k.a. H1.0, H10, H1F0, H1FV)
- Promoter U6
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer TRC-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.05.641622 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX330_H1.0 was a gift from Kazuhiro Maeshima (Addgene plasmid # 247482 ; http://n2t.net/addgene:247482 ; RRID:Addgene_247482) -
For your References section:
Linker histone H1 functions as a liquid-like glue to organize chromatin in living human cells. Shimazoe MA, Huertas J, Phillips C, Ide S, Tamura S, Farr S, Ashwin SS, Sasai M, Collepardo-Guevara R, Maeshima K. Sci Adv. 2026 Apr 10;12(15):eaec9801. doi: 10.1126/sciadv.aec9801. Epub 2026 Apr 8. 10.1126/sciadv.aec9801 PubMed 41950317