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LentiGuide Cyclone-Puro P2A EGFP
(Plasmid #247497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247497 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    LentiGuide Puro-P2A-EGFP (Plasmid #137729)
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 11739
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    puromycin n-acetyltransferase with Cyclone insertion
  • Alt name
    Cyclone-Puro
  • Species
    Synthetic
  • Insert Size (bp)
    1373
  • Mutation
    Cyclone was inserted after Glutamine135 of puromycin n-acetyltransferase to gain acyclovir control of the fusion protein.
  • Promoter EF-1a
  • Tag / Fusion Protein
    • Puro(Cyclone)-P2A-EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiGuide Cyclone-Puro P2A EGFP was a gift from Samie Jaffrey (Addgene plasmid # 247497 ; http://n2t.net/addgene:247497 ; RRID:Addgene_247497)

  • For your References section:

    A portable poison exon for small-molecule control of mammalian gene expression. Hou Q, Oleynikov M, Mei X, Dong L, Hagen T, Jaffrey SR. Nat Methods. 2025 Nov 3. doi: 10.1038/s41592-025-02860-7. 10.1038/s41592-025-02860-7 PubMed 41184552
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