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pLVX_cpCys203
(Plasmid #247756)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247756 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-EF1a-IRES-puro
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 8825
  • Total vector size (bp) 11037
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cpCys203 Cysteine Sensor
  • Insert Size (bp)
    2229
  • Tag / Fusion Protein
    • ECFP and Venus

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX_cpCys203 was a gift from Alan Ashworth (Addgene plasmid # 247756 ; http://n2t.net/addgene:247756 ; RRID:Addgene_247756)

  • For your References section:

    An engineered cysteine sensor optimized for high-throughput screening identifies regulators of intracellular thiol levels. Abrams RPM, Donahue RG, Ma J, Mao Y, Diolaiti ME, Ashworth A. Cell Chemical Biology, 2025 10.1016/j.chembiol.2025.10.006
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