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pET29b_cpCys203
(Plasmid #247771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247771 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET29b
  • Total vector size (bp) 7520
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This plasmid uses a T7 promoter, so expression requires a DE3 strain. For optimal expression, the use of E. coli BL21 Gold (DE3) is recommended.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cpCys203 Cysteine Sensor
  • Insert Size (bp)
    2286
  • Tags / Fusion Proteins
    • ECFP and Venus
    • His6-TEV (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b_cpCys203 was a gift from Alan Ashworth (Addgene plasmid # 247771 ; http://n2t.net/addgene:247771 ; RRID:Addgene_247771)

  • For your References section:

    An engineered cysteine sensor optimized for high-throughput screening identifies regulators of intracellular thiol levels. Abrams RPM, Donahue RG, Ma J, Mao Y, Diolaiti ME, Ashworth A. Cell Chem Biol. 2025 Nov 10:S2451-9456(25)00343-5. doi: 10.1016/j.chembiol.2025.10.006. 10.1016/j.chembiol.2025.10.006 PubMed 41218609
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