Skip to main content
Addgene
Open Menu

pAAV-DIO-MitoDREADD-Gs
(Plasmid #247960)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247960 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.
  • Serotype
    Select serotype for details
  • Pricing
    Select serotype and quantity
  • How this works
    • Place a request for a quantity of 2 (0.2 mL), 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
    • Addgene will quickly confirm that we can produce a high-quality prep for you.
    • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
    • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
    • Learn more about our Packaged on Request Service.

Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MitoDREADD-Gs
  • Alt name
    4MLS-DREADD-Gs
  • Promoter CAG
  • Tag / Fusion Protein
    • 4x COX8 mitochondrial leading sequence, HA (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer ggacggcccttctcctccggg
  • 3′ sequencing primer GTAATCCAGAGGTTGATTATCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-DIO-MitoDREADD-Gs was a gift from Giovanni Marsicano (Addgene plasmid # 247960 ; http://n2t.net/addgene:247960 ; RRID:Addgene_247960)

  • For your References section:

    Potentiation of mitochondrial function by mitoDREADD-G(s) reverses pharmacological and neurodegenerative cognitive impairment in mice. Pagano Zottola AC, Martin-Jimenez R, Lavanco G, Hamel-Cote G, Ramon-Duaso C, Rodrigues RS, Mariani Y, Khan M, Drago F, Jean S, Rio IB, Jimenez-Blasco D, Egana-Huguet J, Eraso-Pichot A, Beriain S, Cannich A, Vidal-Palencia L, Infantino R, Julio-Kalajzic F, Gisquet D, Goncalves A, Al-Younis I, Baussan Y, Duvezin-Caubet S, Devin A, Soria-Gomez E, Puente N, Bolanos JP, Grandes P, Pouvreau S, Busquets-Garcia A, Marsicano G, Bellocchio L, Hebert-Chatelain E. Nat Neurosci. 2025 Sep;28(9):1844-1857. doi: 10.1038/s41593-025-02032-y. Epub 2025 Aug 11. 10.1038/s41593-025-02032-y PubMed 40790270
Related items: