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pART7::CLOMELEON
(Plasmid #247987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247987 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pART7
  • Backbone manufacturer
    Andrew Gleave
  • Backbone size w/o insert (bp) 5042
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
  • Species
    Synthetic
  • Insert Size (bp)
    1508
  • Mutation
    CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T, V163A, N164H, H231L; YFP-variant: S65G, S72A, K79R, T203Y, H231L
  • Promoter Cauliflower Mosaic Virus 35S promoter (GenBank AJ007625), without artificial 40bp insert
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer CAACCACGTCTTCAAAGC
  • 3′ sequencing primer GGATCTGAGCTACACATGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pART7::CLOMELEON is a pART7 vector with the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) integrated between EcoRI and HindIII restriction sites. See the Supplemental Document under Resource Information for more details, including annotated maps.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pART7::CLOMELEON was a gift from Christoph Plieth (Addgene plasmid # 247987 ; http://n2t.net/addgene:247987 ; RRID:Addgene_247987)

  • For your References section:

    Salt stress-induced chloride flux: a study using transgenic Arabidopsis expressing a fluorescent anion probe. Lorenzen I, Aberle T, Plieth C. Plant J. 2004 May;38(3):539-44. doi: 10.1111/j.0960-7412.2004.02053.x. 10.1111/j.0960-7412.2004.02053.x PubMed 15086798
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