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pDisplay-hpGRISZ
(Plasmid #248027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248027 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDisplay
  • Backbone size w/o insert (bp) 5054
  • Total vector size (bp) 6602
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hpGRISZ
  • Alt name
    high-performance green indicator for synaptic Zn2+
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.06.680726 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-hpGRISZ was a gift from Huiwang Ai (Addgene plasmid # 248027 ; http://n2t.net/addgene:248027 ; RRID:Addgene_248027)

  • For your References section:

    hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamics. Zhang H, Kumar M, Correia R, Yang S, Huang W, Soares-Cunha C, Cody PA, Zhang Y, Tian X, Zhao S, Drobizhev M, Rodrigues AJ, Tzounopoulos T, Ai H. bioRxiv 2025.10.06.680726 10.1101/2025.10.06.680726
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