Skip to main content
Addgene
Open Menu

pCGT002_nuclear-EGFP
(Plasmid #248084)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248084 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pLX_313-EGFP
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 8800
  • Total vector size (bp) 9800
  • Modifications to backbone
    Removed CAAX box and DD (degron tag), added 2x NLS at N and C termini of fluorophore.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    712
  • GenBank ID
    AFA52654.1
  • Promoter EF1-alpha
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gggggaggggttttatgcgatgg
  • 3′ sequencing primer acaacgggccacaactcctcataa
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.27.661951 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGT002_nuclear-EGFP was a gift from Arjun Raj (Addgene plasmid # 248084 ; http://n2t.net/addgene:248084 ; RRID:Addgene_248084)

  • For your References section:

    Lineage memory shapes viral resistance barriers in human skin. Van Eyndhoven LC, Kinsler G, Zhang J, O'Farrell A, Abderrahim R, Srivastav A, Triandafillou CG, Greco TM, Zaret KS, Cristea IM, Orzalli MH, Drayman N, Singh A, Raj A. bioRxiv [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 10.1101/2025.06.27.661951. 10.1101/2025.06.27.661951 PubMed 40666908
Related items: